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Journal of Nutrition Vol. 114 No. 10 October 1984, pp. 1917-1923
Copyright © 1984 by American Society for Nutrition
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Frequency and Sequence Complexity of Liver Poly(A)+ mRNAs from Rats Fed Fat-Free or High Fat Diets1,2,

C. Elizabeth Castro and J. Sanders Sevall

Department of Cellular and Molecular Biology, Southwest Foundation for Biomedical Research, P.O. Box 28147, San Antonio, TX 78284

We demonstrate by messenger RNA (mRNA) · complementary DNA (cDNA) hybridization that nutrient variation can alter 1) the frequency in which entire classes or families of liver polyadenylated mRNAs [poly(A)+ mRNAs] are present within the cell and 2) the sequence complexity of those transcripts. Feeding a carbohydrate-rich, fat-free diet to rats for 5 days resulted in an increased sequence complexity of rare and moderately abundant liver poly(A)+ mRNAs when compared with poly(A)+ mRNA from rats fed high fat or basal diets. In addition, the frequency of reiteration of abundant sequences increased approximately 10 times compared with the abundant poly(A)+ mRNAs of rats fed high fat or basal diet. The results indicate that the functionality of the template is influenced by nutrient concentrations and may be one means by which complex organisms achieve homeostasis.

KEY WORDS: • poly(A)+ mRNA • complexity • hybridization

1 Presented in abstract form at the 68th Annual Meeting of the Federation of American Societies for Experimental Biology, St. Louis, MO, 1984, Fed. Proc. 43, 594 (abs. 1808), 1984.

2 Supported by grants AM33259 to C. E. C. and AM28252 to J. S. S. from the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases of the National Institutes of Health.

Manuscript received 22 March 1984.




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