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The Effect of Dietary and Sulfur Compounds in Alleviating 3-Methylindole-Induced Pulmonary Toxicity in Goats^1,2,

Department of Nutrition, College of Biological Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1

The present experiment was designed to determine the effect of tissue concentrations of glutathione (GSH) and GSH-S-transferase activity on 3-methylindole (3MI)-induced pulmonary toxicity in vivo. Forty goats were given high protein, normal protein, high cysteine, high sulfate or diethyl maleate (DEM) to vary tissue concentrations of GSH before i.v. infusion of 3MI. The severity of lung lesion was scored. Tissue GSH concentration, GSH-S-transferase activity and cytochrome P-450 content were measured. Compared to goats fed normal protein diet, high cysteine or high sulfate increased the tissue GSH levels and reduced the severity of the lung lesion induced by 3MI. Pretreatment with DEM, by which the tissue GSH was depleted, increased the severity of 3MI-induced lung lesion. Tissue GSH-S-transferase activity was not changed. These results indicate that the tissue concentration of conjugating agents play an important role in 3MI-induced lung disease.

KEY WORDS: • glutathione • glutathione-S-transferase • lung disease • 3-methylindole • dietary protein

¹ Supported by the Natural Sciences and Engineering Research Council Canada and the Ontario Ministry of Agriculture and Food.

² Presented in part at the 25th Annual Meeting of the Canadian Federation of Biological Societies. Edmonton, Alberta, June 14–18, 1982.

³ Present address: Veterinary Physiology and Pharmacology College of Veterinary Medicine, Texas A & M University, College Station, TX 77843.

⁴ Author to whom reprint requests should be addressed.

Manuscript received 28 January 1983.