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Zinc Transport by Brush Border Membrane Vesicles from Rat Intestine^1,2,

Department of Nutrition, Rutgers University, New Brunswick, NJ 08903 and ^* Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611

Zinc uptake into isolated brush border membrane vesicles from rat intestine was investigated by using a rapid filtration technique. Uptake was saturable at 0.2 mM extravesicular zinc, while at 1.0 mM zinc uptake was nonsaturable. ATP and Na⁺ did not stimulate uptake. Some binding of zinc was noted at zero intravesicular space indicating some membrane binding may occur during transport. Initial rates of uptake were linear up to 0.3 and 0.45 mM extravesicular zinc with vesicles prepared from rats fed adequate and deficient amounts of zinc, respectively. Kinetic analysis of uptake data yielded a K_m of 0.38 mM and J_max of 5.4 nmol · minute^-1 · mg protein^-1 with vesicles from zinc-adequate rats. With vesicles from zinc-depleted rats the K_m was 0.44 mM and J_max was 12.0 nmol · minute^-1 · mg protein^-1. These uptake data suggest the brush border membrane transport system for zinc is influenced by zinc status.

KEY WORDS: • nutrient absorption • intestinal absorption • zinc absorption

¹ Supported in part by National Institutes of Health Grant AM31127 from the National Institute of Arthritis, Disbetes, Digestive and Kidney Diseases and ES03103 from the National Institute of Environmental Health Sciences and the New Jersey Agricultural Experiment Station.

² Presented in part at the American Institute of Nutrition Meeting, New Orleans, LA, April 1982, Fed. Proc. 41, 779 (abs.).

³ Present address: Travenol Laboratories, Inc., Nutrition and Flow Control Division, Deerfield, IL 60015

⁴ To whom all inquiries and reprint requests should be addressed.

Manuscript received 31 January 1983.

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