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Effect of Sucrose or Starch Feeding on the Hepatic Mitochondrial Activity of BHE and Wistar Rats^1,2,

Department of Foods and Nutrition, University of Georgia, Athens, GA 30602

The hypothesis that the effects of dietary sucrose on hepatic lipogenesis could be explained by an effect on mitochondrial respiration was tested. Two groups of male BHE and Wistar rats were fed, from weaning, either a 65% starch or a 65% sucrose diet. Rats were killed at 50–60 days of age by decapitation, and hepatic mitochondria were isolated. Simulated state 3 and state 4 respiration with succinate, pyruvate, or -ketoglutarate as substrates was determined as were the activities of the -glycerophosphate and malate-aspartate shuttles. Mitochondria from Wistar rats had higher ADP:O ratios than BHE rats for all three substrates. Respiratory control ratios with succinate or -ketoglutarate as the substrates were affected by both diet and strain. Respiratory control ratios with pyruvate as a substrate were affected by diet such that sucrose feeding lowered the respiratory control ratio in both BHE and Wistar rats. Shuttle activities were not as markedly affected by strain or diet. We conclude that mitochondrial respiration may be more sensitive to dietary manipulation than mitochondrial shuttle activity.

KEY WORDS: • sucrose • mitochondria • BHE strain • liver

¹ Supported by Georgia Agricultural Research Experiment Station Project H635 and National Institutes of Health grant no. AM 21667.

² A preliminary report of these data was presented in poster form at the 1982 Federation of American Societies for Experimental Biology meetings in New Orleans, LA.

Manuscript received 12 October 1982.

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