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Transketolase, Pyruvate and Oxoglutarate Dehydrogenase Activities and [14C]Thiamin Turnover in Tissues of Mice Fed Thiamin-Deficient Diet

Raisa V. Trebukhina, Yurij M. Ostrovsky, Georgij N. Mikhaltsevich, Magdalena G. Velichko and Veniamin N. Tumanov

Division of Metabolic Regulation, Byelorussian SSR Academy of Sciences, 50 Lenin Komsomol Boulevard, Grodno 230009 USSR

A study was made of turnover of [14C]thiamin (5 or 2 µg/mouse) in mice fed a thiamin-deficient diet. Simultaneously the activities of the thiamin-dependent enzymes (transketolase, pyruvate and oxoglutarate dehydrogenases) were measured as an index of efficiency of fulfilling the coenzyme function of the vitamin under conditions of different thiamin status. After [14C]thiamin injections of 5 µg/mouse, kidney, spleen, stomach and pancreas tissue stores turned over completely on day 9, whereas by day 13 this process had not yet been finished in liver, heart and brain. On administration of 2 µg [14C]thiamin/mouse, turnover of the tissue stores proceeded at a slower rate. The tissue transketolase activity decreased after the 2-µg injections as compared to that in the mice administered 5-µg injections. With 2 µg of [14C]thiamin, the pyruvate dehydrogenase activity lowered gradually in all the tissues studied, whereas the oxoglutarate dehydrogenase decreased in liver and kidneys. The pattern of the depression of the thiamin-dependent enzyme activities after the 2-µg [14C]thiamin injections suggests a regularity in the vitamin redistribution in different organs and subcellular fractions.

KEY WORDS: • thiamin deficiency • transketolase • pyruvate dehydrogenase • oxoglutarate dehydrogenase • turnover

Manuscript received 26 August 1982.




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