QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Swanson, C. A. Articles by Levander, O. A. Search for Related Content PubMed PubMed Citation Articles by Swanson, C. A. Articles by Levander, O. A.

Intrinsic Labeling of Chicken Products with a Stable Isotope of Selenium (⁷⁶Se)

Beltsville Human Nutrition Research Center, U.S. Department of Agriculture, Beltsville, MD 20705

Chicken tissues were intrinsically labeled with a stable isotope of selenium (⁷⁶Se) and were evaluated for use in a human feeding study. Laying hens were fed a low Se (0.06 ppm) basal diet for 39 days and then fed the basal diet supplemented with 0.3 ppm enriched ⁷⁶Se (as selenite) for 35 days. Incorporation of ⁷⁶Se into samples was determined by use of a double isotope dilution technique and a combined gas chromatography/mass spectrometry analysis. The ⁷⁶Se content of the basal diet was increased by a factor of 9.7 with the addition of the enriched stable isotope. This maximal level of enrichment was approached in egg yolk (9.5-fold) and liver (9.0-fold). Enrichment was lower in egg white (7.2-fold) and breast meat (5.0-fold). Level of enrichment in a given tissue reflected both the turnover rate of the tissue and its natural selenium content. Selenium-depleted laying hens continuously fed ⁷⁶Se at the 0.3 ppm level produced egg yolks and livers that were enriched sufficiently with the stable isotope for use in a human metabolic study.

KEY WORDS: • stable isotope • selenium • intrinsic labeling

¹ Research Associate, University of California, Berkeley, CA supported by General Cooperative Agreement No. 58-32U4-1-212 with U.S. Department of Agriculture.

² Research Associate, Childrens Hospital, Boston, MA supported by Specific Cooperative Agreement No. 58-32U4-0-127 with U.S. Department of Agriculture.

³ Author to whom requests for reprints should be addressed.

Manuscript received 9 August 1982.