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Influence of Diet or Intrarectal Bile Acid Injections on Colon Epithelial Cell Proliferation in Rats Previously Injected with 1,2-Dimethylhydrazine^1,2,

Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824

The effects of varying colon bile acid concentrations on rat colon epithelial cell proliferation were studied. Bile acid concentrations were altered by intrarectally injecting either deoxycholic or lithocholic acid for 4 weeks or by increasing the dietary fat or fiber (wheat bran, agar, or carrageenan) intake for 4 weeks. 1,2-Dimethylhydrazine (DMH) was s.c. injected into half of the rats 1 week before treatments began. Colon epithelial cell proliferation was measured by [³H]thymidine autoradiography of colon crypts. Rats injected with DMH had more DNA-synthesizing cells per crypt. Neither bile acid injection nor any of the diets altered the number of DNA-synthesizing cells per crypt. DMH injections, deoxycholic and lithocholic acid intrarectal injections, and dietary agar and wheat bran all increased the total number of cells per crypt. High fat diets and dietary carrageenan did not affect cell number. All diets containing fiber lowered total fecal bile acid concentrations, but increasing the fat content of the diet did not affect them. These results indicate that the bile acid injections and dietary agar and wheat bran induce a slight hyperplasia in the colon.

KEY WORDS: • 1,2-dimethylhydrazine • bile acid • diet • colon • cell kinetics • rats

¹ Michigan State Agricultural Experiment Station Journal Article No. 10444. This study was supported in part by Michigan State University Experiment Station project no. 3202.

² This work was presented in part at the 65th annual meeting of the Federation of American Societies for Experimental Biology, Atlanta, GA, 1981; Fed. Proc. 40: 929.

³ Present address: McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53706.

⁴ To whom reprint requests should be sent.

Manuscript received 10 June 1982.