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Journal of Nutrition Vol. 113 No. 2 February 1983, pp. 394-400
Copyright © 1983 by American Society for Nutrition
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Effect of Selenium on the Subcellular Distribution of Glutathione Peroxidase in Rat Liver, Epididymal Fat Pad and Seminal Vesicle1

Sharon A. Meyer2, Richard C. Ewan3 and Donald C. Beitz

Departments of Biochemistry and Biophysics and Animal Science, Iowa State University, Ames, IA 50011

The effect of dietary selenium on the subcellular distribution of selenium-dependent glutathione peroxidase (SeGSH-Px) activity in rat liver, epididymal fat pad, and seminal vesicle was determined. Tissues were fractionated by differential centrifugation, and the subcellular distribution of SeGSH-Px activity was determined by comparison with the distribution of biochemical marker enzymes. Liver SeGSH-Px activity was located in both the cytosol and mitochondria. In epididymal fat pad and seminal vesicle, SeGSH-Px activity was located primarily in the cytosol; association with another subcellular organelle, however, was indicated. In liver and epididymal fat pad, SeGSH-Px activity increased linearly, and, in the seminal vesicle, increased linearly and quadratically, with increasing dietary selenium concentration. Distribution of SeGSH-Px activity among the cellular fractions from the tissues, however, was not affected by dietary selenium supplementation.

KEY WORDS: • glutathione peroxidase • selenium • subcellular distribution

1 Journal Paper No. J-10646 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, Project No. 2238.

2 Present address: NY State College of Veterinary Medicine Dept. of Physiology, Cornell University, Ithaca, NY 14853.

3 To whom reprint requests should be sent.

Manuscript received 13 July 1982.




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