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Radiometric-Microbiologic Assay of Niacin Using Kloeckera brevis: Analysis of Human Blood and Food¹

Division of Radiation Health Sciences, Department of Environmental Health Sciences, The Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205

Kloeckera brevis, a yeast, was used as the test organism for the development of a radiometric-microbiologic (RMA) assay for niacin. The assay was determined to be sensitive to the 2 ng niacin per vial level and specific for the biologically active forms of this vitamin. The method was shown to be simple, accurate, and precise in the analysis of niacin in human blood and food. The application of the radiometric technique eliminates some of the problems encountered with conventional turbidimetric-microbiologic assay.

KEY WORDS: • radiometric • microbiologic • assay • niacin • blood • food

¹ This work was supported by U.S. Department of Agriculture Science and Education Administration Grants No. 78-59-2243-01-013-1 and 59-2243-1-1-667-0 from the Competitive Research Grants Office.

Manuscript received 13 June 1983.

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