QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Green, G. M. Articles by Nasset, E. S. Search for Related Content PubMed PubMed Citation Articles by Green, G. M. Articles by Nasset, E. S.

Role of Dietary Protein in Rat Pancreatic Enzyme Secretory Response to a Meal¹

Bruce Lyon Memorial Research Laboratory, Children's Hospital Medical Center, Oakland, CA 94609 and Division of Gastroenterology and Nutrition, Department of Medicine, University of Texas Health Science Center, San Antonio, TX 78284

The role of dietary protein in the pancreatic enzyme secretory response to complete test meals was studied in rats fitted with chronic bile-pancreatic fistulas. Pancreatic enzyme secretory response was observed by collecting combined bile-pancreatic juice and continuously returning it to the intestine by a streamsplitter, which diverted 5% for assay. All rats were fed ad libitum a 24% casein diet during the recovery period. Four days postoperative, rats were infused intragastrically with 5 g of isonitrogenous, complete test meals containing casein (24%), -protein (soy protein), amino acids patterned after casein or -protein, casein + lima bean trypsin inhibitor (LBTI), and a nitrogen-free diet. Pancreatic enzyme secretion under basal conditions and in response to the test meals was determined. The pancreatic response to test meals containing intact casein as nitrogen source was the same as the response to nitrogen-free test meals, and was only slightly greater (not statistically significantly) than the response to test meals containing amino acids. Test meals containing -protein, which was devoid of soybean trypsin inhibitor activity, were much more potent than corresponding amino acid test meals or nitrogen-free test meals. Addition of LBTI greatly increased the pancreatic response to casein test meals. The results indicate that intragastric infusion of diets containing casein as nitrogen source do not stimulate greater pancreatic enzyme secretion than test meals of nitrogen-free or amino acid diets under normal physiological conditions, and it is proposed that this is a consequence of the large reserve capacity of the exocrine pancreas.

KEY WORDS: • exocrine pancreas secretion • trypsin inhibitors • protein digestion

¹ Supported by U.S. Public Health Service Grant AM 17737, AM 26757, RR 05467, Cystic Fibrosis Foundation Grant G720C, and University of Texas Institutional Grant.

² Reprint requests: Gary M. Green, Ph.D., Department of Medicine, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284.

Manuscript received 20 May 1983.

This article has been cited by other articles:

				M. Yamamoto, J. R. Reeve Jr., and G. M. Green Supramaximal CCK-58 does not induce pancreatitis in the rat: role of pancreatic water secretion Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G964 - G974. [Abstract] [Full Text] [PDF]

				M. Yamamoto, M. Otani, and M. Otsuki A new model of chronic pancreatitis in rats. Am J Physiol Gastrointest Liver Physiol, October 1, 2006; 291(4): G700 - G708. [Abstract] [Full Text] [PDF]

				M. Yamamoto, J. R. Reeve Jr., D. A. Keire, and G. M. Green Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8 Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G866 - G879. [Abstract] [Full Text] [PDF]