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Effect of Maternal Carbohydrate Intake on Mitochondrial Activity and on Lipogenesis by the Young and Mature Progeny^1,2,

University of Georgia, Department of Foods and Nutrition, Dawson Hall, Athens, GA 30602

The influence of maternal dietary sucrose on lipogenesis and on oxygen consumption and ATP production by isolated hepatic mitochondria was studied. During gestation and lactation, female BHE rats were fed either a 65% starch or a 65% sucrose diet. At weaning, male progeny were fed either the diet of their dam or the alternate diet. Rates of oxygen consumption and ATP production from isolated hepatic mitochondria, rates of ³H incorporation into fatty acids, and percent liver lipid were determined at 50 and 150 days of age. Correlation coefficients between mitochondrial function and hepatic lipogenic activity were calculated. Analysis of variance of these data indicated that state IV oxygen consumption was influenced by age and by an interaction of the maternal diet and the diet fed the progeny from weaning. The respiratory control ratio was influenced by the interaction of the maternal diet and the diet fed the progeny and by the interaction of the maternal diet and the age of the progeny when killed. ATP synthesis and recovery of ADP as ATP was influenced by an interaction of all three variables: maternal diet, growth diet and age. In progeny killed at 150 (but not 50) days of age, sucrose feeding increased the incorporation of tritium into fatty acids. Liver lipid levels were influenced by age and diet. Mitochondrial ATP synthesis was negatively correlated with hepatic lipid content, and mitochondrial ATP recovery from ADP was negatively correlated with ³H incorporation and percent liver lipid. These results suggest that alterations in hepatic mitochondrial ATP production may be associated with the increase in hepatic lipid synthesis and serum lipid levels frequently observed in sucrose-fed animals as they age.

KEY WORDS: • maternal diet • sucrose • starch • respiration • ATP synthesis • mitochondria

¹ Supported by Georgia Experiment Station project H635 and National Institutes of Health Grant AM21667.

² A preliminary report of these data was presented at the 1981 FASEB meetings, Atlanta, GA, Fed Proc. 40:870.

Manuscript received 5 April 1983.

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