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The Folacin Contents of Foods as Measured by a Radiometric Microbiologic Method¹

The Johns Hopkins Medical Institutions, 615 North Wolfe Street, Baltimore, MD 21205-2179

A radiometric microbiologic assay for folate was applied to measure food folacin levels. Sixteen different foods were analyzed. The total folacin content of the 16 foods obtained by the radiometric microbiologic method was found to compare well with that obtained by the turbidimetric microbiologic method. The folacin content ranged from 9 ng/ml of frozen reconstituted grape juice to 1920 µg/100 g of the cereal Total. The study showed that the radiometric microbiologic assay can be used to measure folacin content in foods.

KEY WORDS: • food folacin • radiometric microbiologic assay

¹ This work was supported by the Science and Education Administration of the U.S. Department of Agriculture under Grant No. 78-59-2243-0-1-013-1 (formerly Grant No. 5901-0410-8-0013-0) from the Competitive Research Grants Office.

² Present address: University of South Florida, Department of Internal Medicine, Division of Digestive Diseases and Nutrition, 12901 North 30th Street, Tampa, FL 33612.

Manuscript received 7 March 1983.