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NADPH-Dependent Reduction of Cytochrome P-450 in Liver Microsomes from Vitamin C-Deficient Guinea Pigs: Effect of Benzphetamine¹

Department of Pharmacology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190

The effect of dietary vitamin C on the reduction of cytochrome c and cytochrome P-450 by NADPH-cytochrome P-450 reductase was determined in guinea pig liver microsomes. Acute vitamin C deficiency decreased hepatic microsomal cytochrome P-450 content 21% and the rate of cytochrome P-450 reduction 66% without affecting cytochrome c reduction. However, the vitamin status of the animals did not affect the enhancement in cytochrome P-450 reduction produced by the addition of a type I substrate to the reaction mixture. The results suggest that vitamin C deficiency selectively affects the transfer of electrons from NADPH to cytochrome P-450 and that this effect does not result from a change in the spin state of cytochrome P-450.

KEY WORDS: • vitamin C • liver microsomes • NADPH-cytochrome P-450 reductase

¹ Supported by National Institutes of Health Grant GM 26480.

Manuscript received 16 April 1982.