QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hung, S. S. O. Articles by Slinger, S. J. Search for Related Content PubMed PubMed Citation Articles by Hung, S. S. O. Articles by Slinger, S. J.

Uptake, Transport and Distribution of DL--Tocopheryl Acetate Compared to D--Tocopherol in Rainbow Trout (Salmo gairdneri)¹

Silas S. O. Hung^2,3,*, Thomas W. Moon, John W. Hilton^* and Stanley J. Slinger^*

^* Department of Nutrition, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5

The appearance of radioactivity after the oral administration of 3 µCi D--[5-methyl-³H]tocopherol and 10 µCi DL--[3',4'-¹⁴C]tocopheryl acetate in plasma, liver, kidney, spleen and heart of rainbow trout showed an exponential increase up to 32 hours, followed by a plateau or slight decline from 32 to 64 hours. Radioactivity in the skeletal muscle increased exponentially up to 8 hours followed by a slower linear increase up to 64 hours. Comparisons of plasma ³H and ¹⁴C radioactivity suggested that the uptake of D--tocopherol (EOH) was 6 to 18 times greater than DL--tocopheryl acetate (EAc) in the first 4 hours and 2 to 3 times greater between 8 and 64 hours. At the plateau, the amount of ³H and ¹⁴C radioactivity incorporated per unit wet weight of tissue decreased in the order liver > kidney > plasma > spleen > heart » skeletal muscle. More than 87% of the ³H and ¹⁴C radioactivity after 16 hours was found to be free -tocopherol in both plasma and liver. The radioactively labeled vitamins were bound primarily to plasma low-density lipoprotein (density 1.015 to 1.085). These studies support the hypothesis that the uptake, transport and distribution of EAc after hydrolysis in the gastrointestinal tract of trout follows a pattern similar to that of EOH.

KEY WORDS: • vitamin E • uptake • tissue distribution • transport • trout

¹ This work was financed by two grants from the National Sciences and Engineering Research Council Canada to TWM and SJS, and a grant to JWH from the Ontario Ministry of Agriculture and Food.

² Present address: Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5.

³ To whom reprint requests should be sent.

Manuscript received 23 February 1982.