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Actual Nitrogen Deposition in Mature Adult Rats Fed Moderate to High Protein Diets

Department of Biochemistry, College of Medicine, National Taiwan University, Taipei, Taiwan 100 Republic of China

Comparison between the positive N balance data and the actual N deposition in the same adult animals was made. In experiment 1, 24 mature 9-monthold male rats were divided into three groups. One group was killed on day zero and served as the control. The two other groups were fed 25 or 49% casein diet for 8 weeks. Body weight was kept relatively constant by regulating the dietary intake. The N balance study (including body hair N loss) was conducted thrice intermittently, and body N was determined at the end of the experiment. It was found that the mean total-body N of the 49% casein group was 967 mg higher than the 25% casein group and 885 mg higher than the day-zero control group (P < 0.01). The calculated N retention during the experimental period was 1876 mg for the 49% casein group. In experiment 2, a similar study was conducted except that the 25 and 49% casein diets were fed ad libitum, and body weight gain was allowed. The total-body N values of both test groups at the end of the feeding period were very close to each other, but significantly higher than that of the day-zero control group (P < 0.01).

KEY WORDS: • nitrogen balance • adult rat • high protein diet • body nitrogen deposition

¹ Present address: Department of Microbiology, College of Science, Soochow University, Wal Shuang Hal, Shih Lin, Taipei, Taiwan, Republic of China.

² Author to whom reprint requests should be sent.

Manuscript received 13 November 1981.

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