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Development of Gluconeogenesis in Isolated Hepatocytes from Fasting or Suckling Newborn Pigs¹

Jean Paul Pegorier^2,°, Pierre Henri Duee, Jean Girard and Jean Peret^°

^° Centre de Recherches sur la Nutrition du C.N.R.S., 9, rue Jules Hetzel, 92190 Meudon-Bellevue, France, and Laboratorie de Physiologie du Développement du Collège de France, 11, place Marcellin Berthelot, 75231 Paris, Cedex 05, France

The rates of glucose production from various substrates were investigated in isolated hepatocytes from fasting and suckling newborn pigs from birth to 48 hours of life. They were quantitatively compared to the rate of glucose production measured in 15-day-old suckling and fasting piglets. The data indicate that the rates of gluconeogenesis from each gluconeogenic precursor (lactate, pyruvate, alanine, glycerol, dihydroxyacetone and galactose) were two- to threefold higher in suckling than in fasting newborn pigs. The rates of glucose production from various gluconeogenic precursors were similar in suckling newborn pigs and in 15-day-old fasting piglets. The difference in the capacity for gluconeogenesis in isolated hepatocytes from fasting and suckling newborn pigs could explain why hypoglycemia develops during fasting in newborn pigs whereas suckling neonates remain normoglycemic.

KEY WORDS: • isolated hepatocytes • fasting • suckling newborn pigs • 15-day-old piglets • gluconeogenesis

¹ This work was supported by the Institut National de la Santé et de la Rocherche Médicale (RCP 50.85.38).

² To whom reprint requests should be sent.

Manuscript received 15 October 1981.