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Journal of Nutrition Vol. 112 No. 3 March 1982, pp. 574-583
Copyright © 1982 by American Society for Nutrition
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Estimation of Plasma Histidine Flux in Cows1

Michael L. Bruss and Arthur L. Black

Department of Physiological Sciences University of California Davis, CA 95616

A tracer method is described for estimating the plasma amino acid flux in animals during steady state, and its application is illustrated using lactating cows that were injected intravenously with 14C- or 3H-labeled histidine. The method involves an extension of the occupancy principle in which the fraction of tracer recovered in a metabolic sink, casein or hemoglobin, provides the basis for calculating the amino acid flux. The plasma histidine flux was 25 g/day based on recovery of istope in milk casein and 31 g/day based on recovery of isotope in hemoglobin.

KEY WORDS: • histidine flux • cows • casein • hemoglobin

1 Supported in part by Public Health Service (NIH) grants GM-633 and HD02375.

Manuscript received 25 August 1981.




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