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Effects of Dietary Selenium and Vitamin E on Covalent Binding of Aflatoxin to Chick Liver Cell Macromolecules¹

Institute of Health, Chinese Academy of Medical Sciences, Beijing, The People's Republic of China in cooperation with Dept. of Poultry and Avian Sciences, Cornell University, Ithaca, NY 14853 and Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853

Day-old single comb white Leghorn chicks of both sexes maternally depleted in selenium (Se) and vitamin E (VE) were fed a low Se and VE-free semipurified basal diet or that diet supplemented with graded levels of Se (0.2–20.0 ppm as Na₂SeO₃) or VE (100 IU/g as all-rac--tocopheryl acetate), or both. At 14 days of age, chicks were given 1 mg/kg [³H] aflatoxin B₁ (AFB₁) i.p. and killed either 2 or 24 hours later. Covalent binding of AFB₁ to liver DNA and RNA in chicks fed the basal diet was significantly greater than in chicks supplemented with Se or VE, or both. Phenobarbital treatment prior to administration of AFB₁ decreased adduct formation in most groups, and abolished differences in adduct formation due to diet. These results suggest that combined Se-VE deficiency enhances activation or inhibits detoxification of AFB₁ in vivo.

KEY WORDS: • selenium • vitamin E • aflatoxin adducts

¹ Supported by USPHS Grants 5 PO1 CA26755 and 1 RO1 ESO1354, Hatch Project NYC-399412, and a grant from the Herman Frasch Foundation.

² Reprint requests should be addressed to T. Colin Campbell, Division of Nutrition Sciences, Cornell University, Ithaca, NY 14853.

Manuscript received 7 July 1981.

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