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Endotoxin-Induced Changes in Copper and Zinc Metabolism in the Syrian Hamster^1,2,

Department of Nutrition, Rutgers University, New Brunswick, NJ 08903

The temporal response of zinc and copper metabolism to endotoxin administration was examined in Syrian hamsters over a 144-hour period. Serum copper was significantly elevated at 12, 24 and 72 hours after endotoxin, whereas serum zinc was reduced 4–48 hours after treatment. A brief elevation (8 hours) in liver copper concentration and a sustained (72 hours) increase in liver zinc concentration were also observed. The amount of zinc associated with liver metallothionein (MT) progressively increased with time, to a plateau by 24 hours and persisted at the elevated level until 72 hours after endotoxin treatment. In vitro translation of poly (A)⁺ RNA from liver polyribosomes showed that following endotoxin treatment MTmRNA activity was maximally elevated 6 hours after endotoxin administration and remained elevated 24 and 48 hours thereafter. Slab gel electrophoresis of serum proteins indicated changes in a stainable protein comigrating with purified ceruloplasmin after endotoxin administration. Pooled gingival tissue from endotoxin-treated hamsters demonstrated a consistently elevated copper content 12–144 hours after treatment. Endotoxin isolated from Bacteroides melaninogenicus was more effective in elevating gingival and serum copper and gingival zinc than Escherichia coli endotoxin. It was concluded that endotoxin administration elicits responses that result in enhanced metallothionein mRNA activity. In addition, Cu and Zn concentrations in serum, liver and gingival tissue are influenced by different endotoxins to different degrees.

KEY WORDS: • endotoxin • metallothionein • zinc • copper • liver

¹ Supported in part by National Institutes of Health Grants AM 18555 from the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases and DE 04606 from the National Institute of Dental Research and as Project 14112 of the New Jersey Agricultural Experiment Station.

² Presented in part at the American Institute of Nutrition Meeting. Atlanta, GA, April 1981. Fed. Proc. 40, 938 (abs.).

³ Present address: Department of Periodontics, University of Texas Health Science Center, San Antonio, TX 78234.

⁴ Present address: Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611.

⁵ Present address: Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226.

⁶ To whom all inquires and reprint requests should be addressed.

Manuscript received 7 July 1982.

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