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Effect of Unpalatable Diets, Food Restriction and Saccharin-Adulterated Diet on Tryptic, Chymotryptic, and Amylolytic Activity in Pancreas, Intestine and Feces of Rats^1,2,

Michael Naim^*, Joseph G. Brand^,, and Morley R. Kare

^* Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel and Monell Chemical Senses Center and Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104

Growing rats were fed either 10 or 17% protein (casein) diets whose taste was changed daily by addition of aversive stimuli. Under these circumstances food intake and body weight gain were reduced compared with controls fed unadulterated diets. More amylolytic activity per gram pancreas was found in rats fed a 10% protein, aversive diet and in their pair-fed controls fed restricted amounts of an unadulterated diet compared with ad libitum controls. More amylolytic activity per gram chyme of small intestine was also found in rats fed less than ad libitum amounts of a 10% protein diet. The chymotrypsin activity in the pancreas of rats fed a 17% protein, aversive diet was greater than in that of control animals or that of the pair-fed animals. The 2- to 10-fold higher proteolytic activity found in the small intestine and especially in the large intestine and feces of rats fed the aversive diet was specificially related to the adulteration of the diet with sodium saccharin. Feeding a diet containing sodium saccharin led to lower cecal pH. Under conditions of the present experiments, changes in digestive enzyme activity are not directly related to the aversive taste of the diet.

KEY WORDS: • aversive taste • unpalatable diets • food restriction • saccharin • trypsin • chymotrypsin • amylase

¹ This investigation was supported in part by a grant from the Central Fund of the Hebrew University, and by BRSG Grant SO7 RR 05825-05 awarded to the Monell Chemical Senses Center by the Biomedical Research Support Grant Program, Division of Research Resources, National Institutes of Health.

² Preliminary results of this study were presented in the ECRO-ISOT Joint Congress on Chemoreception, Noordwijkerhout, Holland, July, 1980. Abstract published in 1980 in Olfaction and Taste VII, p. 426, Information Retrieval Ltd., London.

Manuscript received 20 April 1982.