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The Biological Activity of 25-Hydroxycholecalciferol and 1,25-Dihydroxycholecalciferol for Rainbow Trout (Salmo gairdneri)^1,2,

Department of Nutrition, College of Biological Science, University of Guelph, Guelph, Ontario N1G 2W1 Canada ^* Research Institute, The Hospital for Sick Children, Toronto, Ontario M5G 1X8 Canada

Dietary 25-hydroxycholecalciferol (25-OH-D₃) and 1,25-dihydroxycholecalciferol [1,25-(OH)₂D₃] showed vitamin D activity in rainbow trout. However, inclusion of dietary cholecalciferol (vitamin D₃ or D₃), ergocalciferol (vitamin D₂ or D₂), 25-OH-D₃ or 1,25-(OH)₂D₃ did not result in the presence of detectable levels of vitamin D or 25-OH-D in the blood plasma of the fish. Fish fed the diet devoid of vitamin D over an extended period of time showed symptoms of a droopy-tail or "lordosis-like" syndrome that appeared to be related to muscle weakness since x-ray examination indicated no abnormality in vertebral development. The requirement for vitamin D as cholecalciferol was in excess of 1600 IU/kg diet and may be as high as, or higher than 2400 IU/kg diet.

KEY WORDS: • vitamin D • vitamin D metabolites • requirements • trout

¹ This study represents part of the dissertation submitted by B. J. Barnett in partial fulfillment of the requirements for the M.Sc. degree, University of Guelph.

² This study was financed by the Natural Sciences and Engineering Research Council Canada, Fisheries, Branch, Fisheries and Environment Canada, Fisheries and Marine Service, Ontario Ministry of Natural Resources, Sport Fisheries Branch and the Ontario Ministry of Agriculture and Food.

³ To whom reprint requests should be sent.

Manuscript received 6 January 1982.