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Journal of Nutrition Vol. 112 No. 10 October 1982, pp. 1881-1891
Copyright © 1982 by American Society for Nutrition
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Localization of Retinol (Vitamin A) in Rat Testes1

Shakuntala U. Rajguru*, Yuan-Hsu Kang{dagger} and Balwant S. Ahluwalia2,*

* Department of Obstetrics and Gynecology {dagger} Department of Zoology and Pharmacology, College of Medicine, Howard University, Washington, DC 20059

The purpose of the study was to determine the intracellular location of exogenously injected labeled vitamin A (retinol) in rat testes by light and electron microscopic autoradiography. Tritium-labeled vitamin A mixed in vitamin A-free rat serum was injected intracapsularly in one testis in adult rats. At intervals of 30 minutes, 2 hours, and 4 hours following injection the treated testes were removed and processed for autoradiography. The results show that in the testes vitamin A is localized at three sites: within the macrophages of the interstitial tissue, in the lipid droplets of the Sertoli cells, and in the spermatids in association with Golgi saccules. To confirm the autoradiographic localization of vitamin A, the cellular retinol-binding protein (CRBP) assay demonstrates the presence of CRBP in the interstitial tissue and seminferous tubules. The results of the study suggest a possible role of vitamin A in spermiogenesis in rat testes.

KEY WORDS: • vitamin A • Sertoli cell • spermatid • cell retinol-binding protein

1 This study was supported by National Institutes of Health grant HD 13641.

2 To whom reprint requests should be sent: Department of Obstetrics and Gynecology, College of Medicine, Howard University, Washington, DC 20059.

Manuscript received 8 February 1982.




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