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Purine Availability and Metabolism in Dogs Fed Single-Cell Protein or RNA

Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Universität München, Munich, West Germany

For the evaluation of single-cell protein (SCP) the porportion of dietary purines made available by digestion and absorption was estimated with dogs (Dalmatian) in total urine collection experiments. The animals received a low purine control diet with 25% casein, diets with 25–100% replacement of casein by a bacterial SCP grown on methanol, and the control diet plus yeast RNA purine equivalent to SCP diet. Nucleic acids, bases, and purine metabolites were determined by reversed-phase high-pressure liquid chromatography. The quantitative influence of dietary purines (x) on renal purine excretion (y) was described by the equation (millimoles per day) y = 0.57x + 3.52 (r = 0.955) indicating the endogenous excretion rate of 3.5 mmol/day and the metabolic availability of 57% of ingested purines. As the ratio of urate to allantoin increased with purine intake, individual equations were established for both metabolites. On the control diet + RNA and SCP diet (100% replacement for casein), purine intakes of 20.0 and 18.2 mmol/day resulted in purine excretion values of 7.3 and 11.4 mmol/day (P < 0.05). The proportion of free bases in the latter diet appeared as the main reason for this difference. Other influences are discussed.

KEY WORDS: • purine metabolism • single-cell protein • nucleic acids • dog

¹ Address reprint requests to: Institute of Animal Physiology, Veterinärstrasse 13, D-8000 München 22, West Germany (F.R.G.).

Manuscript received 1 March 1982.

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