Journal of Nutrition Animal Diets/Enrichment Products...

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 111 No. 9 September 1981, pp. 1556-1567
Copyright © 1981 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cowey, C. B.
Right arrow Articles by Knox, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cowey, C. B.
Right arrow Articles by Knox, D.

Tissue Distribution, Uptake, and Requirement for {alpha}-Tocopherol of Rainbow Trout (Salmo gairdneri) Fed Diets with a Minimal Content of Unsaturated Fatty Acids

Colin B. Cowey, John W. Adron, Michael J. Walton, John Murray, Arthur Youngson and David Knox

N.E.R.C. Institute of Marine Biochemistry, St. Fittick's Road, Aberdeen, AB1 3RA, U. K. and Torry Research Station, Abbey Road, Aberdeen AB9 8DG, U. K.

The metabolism of and requirement for {alpha}-tocopherol in rainbow trout fed diets containing 1% linolenic acid as sole source of unsaturated fat and graded levels of tocopherol (0.06–10 mg/100 g) were examined. Fish grew 5-fold over a 16-week period. In liver, tocopherol was concentrated in mitochondria with little in cytosol. Orally administered [3H]-tocopherol was rapidly taken up by plasma and liver but uptake into erythrocytes and white muscle was much slower; in most tissues radioactivity reached a plateau after about 3 days but in red muscle radioactivity increased over a 10-day period. Activities of enzymes that prevent free radical initiated tissue damage did not change in tocopherol deficiency. Tocopherol-deficient trout had no gross or subcellular pathologies even though liver and muscle were severely depleted of the vitamin. Ascorbic acid-stimulated lipid peroxidation in liver organelles indicated a tocopherol requirement of 2—3 mg/100 g diet; the molar ratios of polyunsaturated fatty acids to tocopherol in livers of trout fed diets lacking or supplemented with tocopherol (10 mg/100 g) were 980 and 170, respectively.

KEY WORDS: • tocopherol • tissue distribution • absorption • trout

Manuscript received 27 March 1981.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]