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Fatty Acid Synthesis from Lactate in Growing Cattle¹

Department of Biochemistry and Biophysics and Nutritional Physiology Group, Department of Animal Science, Iowa State University, Ames, IA 50011

Rates of fatty acid synthesis from lactate and acetate and activities of selected lipogenic and NADPH-generating enzymes were determined in subcutaneous, intermuscular and intramuscular adipose tissues of cattle that were 11–19 months of age. Fatty acid synthesis from lactate and acetate increased from 11 to 13 months of age in subcutaneous and intermuscular adipose tissues; synthesis from lactate increased until 17 months of age. In intramuscular adipose tissue, synthesis from lactate also increased until 17 months of age while that from acetate continually increased. Activities of NADPH-generating enzymes increased in all three fat depots from 11 to 13 months in age, and little change occurred thereafter. Acetyl-CoA carboxylase activity was constant over entire growth period in all depots. Activity of ATP-citrate lyase increased from 11 to 13 months of age in subcutaneous and intermuscular adipose tissues, but did not increase until 19 months of age in intramuscular adipose tissue. In all cases, activities of ATP-citrate lyase were sufficient to support synthesis from lactate; therefore, lactate conversion to fatty acids in bovine adipose tissues seems to use the citrate cleavage pathway for generation of cytosolic acetyl-CoA.

KEY WORDS: • lactate • fatty acid synthesis • cattle • citrate cleavage pathway

¹ Journal Paper No. J-10042 of the Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames; Project 2187. Supported in part by grants from the Iowa Beef Industry Council and Biomedical Research Support Grant No. 5 SO5 PRO7034.

² Present address: Department of Pathology, U.A.B. Medical Center, Birmingham, AL 35294.

³ Present address: Department of Animal Industry, University of Puerto Rico, Mayaguez, PR 00780.

⁴ Present address: Department of Animal Science, Auburn University, Auburn, AL 36830.

Manuscript received 17 November 1980.

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