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Journal of Nutrition Vol. 111 No. 12 December 1981, pp. 2236-2239
Copyright © 1981 by American Society for Nutrition
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Comparative Measurement of Zinc Absorption in Rats with Stable Isotope 70Zn and Radioisotope 65Zn

Grace S. Lo, Fred H. Steinke, Bill T. G. Ting, Morteza Janghorbani and Vernon R. Young

Central Research, Ralston Purina Company, Checkerboard Square, St. Louis, MO 63188 and Department of Nutrition and Food Science, Laboratory of Human Nutrition, Nuclear Reactor Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139

The use of stable isotopes in a study on zinc bioavailability was evaluated in rats by comparing the zinc absorption of a stable isotope 70Zn and a radioisotope 65Zn simultaneously. The rats of low zinc status were obtained by feeding 1.2% phytic acid from sodium phytate and 12 ppm zinc from zinc sulfate in a semipurified diet for 14 days. The rats were then randomly assigned into five groups of ten rats. The rats were intubated with a test diet mixture with a phytate: zinc molar ratio of 0, 6.25, 12.5, 25 or 50 along with one µCi of 65Zn and 0.02 mg of 70Zn in 2.5 ml of intubating solution. Four hours after the intubation, rats were allowed to consume the diets having the same phytate:zinc ratio as used for intubation ad libitum. Feces were collected every 24 hours for a total of 168 hours. The 65Zn and 70Zn content of diets and fecal samples were determined and the percent of zinc absorption was calculated. The results of this study indicated that the zinc absorption determined by these two isotopes were highly correlated with a correlation coefficient ratio of 0.914

KEY WORDS: • stable isotopes • radioisotopes • zinc bioavailability • zinc absorption

Manuscript received 21 September 1981.




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