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Journal of Nutrition Vol. 111 No. 11 November 1981, pp. 1869-1875
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Radiometric-Microbiologic Assay of Vitamin B-6: Application to Food Analysis1

Tomas R. Guilarte2, Barry Shane and Patricia A. Mcintyre

Division of Radiation Health Sciences, Department of Environmental Health Sciences and the Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore MD 21205

A radiometric microbiologic assay for vitamin B-6 was applied to food analysis. The method was shown to be specific, reproducible and simpler than the standard turbidimetric microbiologic technique. The analysis of seven commercially available breakfast cereals was compared to a high performance liquid chromatography method. Three out of the seven cereals agreed when assayed with both methods (P > 0.1). Four cereals, however, differed in value considerably (P < 0.05). Further studies are required to determine whether these differences were due to different extraction procedures used. The study showed that the new radiometric-microbiologic method can be used to measure total vitamin B-6 or, combined with a column separation procedure, to analyze for specific forms of the vitamin.

KEY WORDS: • vitamin B-6 • radiometric

1 Supported by USDA Science and Education Administration grant 78-59-2243-0-1-013-1 from the Competitive Research Grants Office. Also supported by a graduate student (Dr. Guilarte) stipend from Cooperative Research 12-14-1001-1222 between Department of Environmental Health Sciences, Division of Radiation Health Sciences, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, and USDA Nutrient Composition Laboratory, Nutrition Institute, Beltsville, MD.

2 To whom reprint requests should be sent.

Manuscript received 16 March 1981.




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