QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tepperman, H. M. Articles by Tepperman, J. Search for Related Content PubMed PubMed Citation Articles by Tepperman, H. M. Articles by Tepperman, J.

Effects of High Glucose and High Lard Diets on the Activities of Rat Liver Glycosyltransferases

Department of Pharmacology, State University of New York, Upstate Medical Center, Syracuse, NY 13210

The activities of several enzymes involved in glycoprotein synthesis were measured in the livers of rats (L) fed diets with 67% of calories as lard and compared with those of rats (G) fed 67% glucose diets for 5–9 days. Glucosamine synthetase activity was not influenced by diet, but the activities of UDP-N-acetylglucosaminyl, galactosyl and sialyltransferases were significantly greater in the livers of the rats fed the glucose diet than in L rat livers. The content of UDP-N-acetylglucosamine was also higher in G livers than in the L group. Activities of glycosidases and of sugar nucleotide pyrophosphatases and phosphatases were the same on both diets. Serum total hexosamine was higher in L than in G rats. These findings are discussed in relation to earlier reports that liver plasma membranes from G rats contained more glycoprotein carbohydrate than L membranes.

KEY WORDS: • glycoprotein synthesis • glycosyltransferases

¹ Supported by National Institute of Arthritis, Metabolic and Digestive Diseases, AM 05410.

Manuscript received 3 February 1981.