Journal of Nutrition

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 111 No. 1 January 1981, pp. 154-165
Copyright © 1981 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stein, T. P.
Right arrow Articles by Mullen, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stein, T. P.
Right arrow Articles by Mullen, J. L.

Protein and Fat Metabolism in Rats during Repletion with Total Parenteral Nutrition (TPN)1

T. P. Stein2, G. P. Buzby, M. J. Leskiw, A. R. Giandomenico and J. L. Mullen

Surgical Research Laboratories, Graduate Hospital, and Department of Surgery, University of Pennsylvania, Philadelphia, PA 19146

We investigated the effect of non-protein calorie source on the repletion by total parenteral nutrition (TPN) of protein-depleted rats. Rats were depleted by feeding them a protein free diet for 4 weeks. Control (non-depleted) and depleted rats were given one of three isocaloric, isonitrogenous TPN regimens for 6 days. They differed in the proportion of non-protein calories given as glucose and fat: diet 1, 100% glucose; diet 2, 75% glucose, 25% fat; and diet 3, 100% fat. The liver, skeletal muscle and whole body protein synthesis and breakdown rates were measured by using [15N]glycine as a tracer. The fatty acid distribution in the liver was measured by gas chromatography-mass spectrometry. Results: (a) Omission of fat leads to a decrease in the fraction of linoleate in the liver. (b) Giving all the non-protein calories as a fat emulsion causes fatty acid accumulation in the liver and a significant increase in the liver protein fractional synthesis rate in the control rats and killed about half the depleted rats. (c) For TPN regimens 1 and 2 both the muscle and whole body synthesis rates were increased during repletion. (d) Of the three TPN regimens, the second one which contained both fat and glucose was superior to the other two.

KEY WORDS: • total parenteral nutrition • protein synthesis • fat

1 Supported by USPHS Grant # AM 16658 and the McCabe Fund of the Department of Surgery of the University of Pennsylvania.

2 To whom reprint requests should be sent: Surgical Research Laboratories, Graduate Hospital, University of Pennsylvania, 19th and Lombard Sts., Philadelphia, PA 19146.

Manuscript received 8 May 1980.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]