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Effects of Iron on the Anti-Coli Capacity of Sow's Milk in Vitro and in Ligated Intestinal Segments^1,2,3,

Purdue University, Department of Animal Sciences, Lilly Hall, West Lafayette, IN 47907

The effect of iron on the ability of sow's milk to suppress Escherichia coli was examined both in vitro and in ligated intestinal segments of neonatal pigs. Sow's milk from day 7 of lactation was diluted with NaHCO₃/KH₂ PO₄ and divided into four treatment groups: (1) diluted milk; (2) diluted milk + ferric citrate; (3) diluted milk heated at 100° for 20 minutes, and (4) treatment 3 + ferric citrate. Treatment groups were tested for antibacterial activity in vitro after inoculation of 10⁴ Escherichia coli (strain 263); counts after a 3-hour incubation were: 721, 9,290, 11,400 and 9,680 for treatments 1–4, respectively. The same four treatments were tested in ligated intestinal segments of 9-day-old pigs with or without the addition of 10⁴ E. coli. The average total E. coli count (x 10⁵) per centimeter of intestine for 16 pigs after 3 hours of incubation were: 11, 50, 84 and 249 for treatments 1–4, respectively, without additional E. coli and 14, 110, 314 and 535 with additional E. coli. The effects of exogenous iron on the in vitro anti-coli capacity of sow's milk were also examined at 3-day intervals throughout the first 21 days of lactation. Iron had a significant detrimental effect on the anticoli capacity of sow's milk from days 6 to 18 of lactation. Iron did not have an effect on the anti-coli capacity of colostrum, which exhibited bactericidal activity, or on milk from the 21st day of lactation, which allowed good E. coli growth. Results from these studies suggest that iron reduces the capacity of sow's milk to repress E. coli concentrations both in vitro and in vivo.

KEY WORDS: • iron • Escherichia coli • milk • pigs • immunity

¹ Journal Paper No. 7870, Purdue University Agricultural Experiment Station.

² Supported in part by a grant from the National Pork Producers Council.

³ Parts of this work were presented at March 1979 FASEB meetings. Fed. Proc. 38, 763, 1979.

⁴ Present address: Department of Poultry Science, Rice Hall, Cornell University, Ithaca, NY 14853.

⁵ To whom reprint requests should be sent.

Manuscript received 4 December 1979.