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Use of Deuterium Oxide for the in Vivo Prediction of Body Composition in Female Rats in Various Physiological States¹

Animal Science Department, North Carolina State University, Raleigh, NC 27650

Deuterium oxide (D₂O) was used to estimate total body water (TBW) and subsequently to predict the body composition of female rats at breeding, after pregnancy and after lactation as influenced by dietary manipulation. The correlation between D₂O space (grams) and body water (grams) was 0.893 (P < 0.01). Deuterium oxide space accounted for 70% of the variance in predicting the weight of TBW. Fixed effects (pregnancy, lactation, feeding levels and two-way interactions) accounted for an additional 10% (P < 0.05) of the variance. The correlation between TBW in grams (predicted from D₂O space, grams) and total body protein (grams) was 0.700 (P < 0.01). Total body water accounted for 49% of the variance in total body protein. Fixed effects accounted for an additional 21% (P < 0.05) of the variance in total weight of body protein. The correlations between D₂O space (%) and body water (%) and between D₂O space (%) and body fat (%) were 0.786 (P < 0.01) and -0.758 (P < 0.01), respectively. The accuracy of estimation of body water (%) and body fat (%) from D₂O space was not significantly improved (P > 0.05) when the fixed effects (pregnancy, lactation, feeding levels and two-way interactions) were included in the model.

KEY WORDS: • deuterium oxide • prediction • in vivo • body composition

¹ Paper No. 6208 of the Journal Series of the North Carolina Agr. Res. Serv., Raleigh. Use of trade names in this publication does not imply endorsement of the products mentioned.

² Present address: Department of Animal Science, Kasetsart University Bangkhen, Bangkok, 9 Thailand.

³ Supported in part by the Rockefeller Foundation, 1133 Avenue of the Americas, New York, NY 10036.

⁴ To whom reprint requests should be sent.

Manuscript received 19 November 1979.