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Metabolic Responsiveness of Different Size Adipocytes to Fasting and Refeeding in the Pig¹

Department of Animal Science, Andrew Boss Laboratory—Meat Science, University of Minnesota, St. Paul, MN 55108

The effects of fasting (F) and fasting-refeeding (FR) on [U-¹⁴C]glucose and [I-¹⁴C]palmitate metabolism were examined in pig middle subcutaneous (MSQ) adipocytes fractionated into 20–63, 63–102 and 102–153 µm diameter ranges. Fasting caused a similar decrease in de novo glyceride fatty acid (GFA) and glyceride-glycerol (GG) synthesis from glucose among adipocytes of different size. After refeeding for 2 days, glucose metabolism significantly increased in all fractions, however, the increase did not reach non-fasting levels. There was a disparate increase in glucose metabolism among adipocyte fractions after refeeding. Adipocytes 102–153 µm in diameter had a larger increase in glucose metabolism than smaller adipocytes. Fasting suppressed palmitate esterification rates in 20–63 and 63–102 µm fractions. Esterification by adipocytes 102–153 µm in diameter was not diminished after fasting. An "overshoot" in esterification by adipocytes 63–102 and 102–153 µm in diameter relative to control adipocytes was observed after refeeding; no "overshoot" occurred in 20–63 µm adipocytes. These results indicate that adipocytes of different size have markedly different capacities for substrate utilization after F or FR as well as different metabolic adaptations to F or FR.

KEY WORDS: • pigs • lipogenesis • fatty acid esterification • fasting • refeeding • adipocyte size

¹ Paper No. 11,182. Scientific Journal Series, Minnesota Agricultural Experiment Station, St. Paul, MN.

² Present address: Department of Dairy and Animal Science, The Pennsylvania State University, University Park, PA 16802.

³ To whom reprint requests should be sent.

Manuscript received 13 September 1979.