QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gabrielsen, B. O. Articles by Opstvedt, J. Search for Related Content PubMed PubMed Citation Articles by Gabrielsen, B. O. Articles by Opstvedt, J.

A Biological Assay for Determination of AvailabilityoÃSeleniumfor Restoring Blood Plasma Glutathione Peroxidase Activity in Selenium-Depleted Chicks^1,2,

Norweigan Herring Oil and Meal Research Institute, 5033 Fyllingsdalen, Bergen, Norway

This study was undertaken to develop an assay for the determination of biological availability of selenium. In a preliminary experiment, commercial male, White Leghorn chicks had significantly reduced glutathione peroxidase activity in blood plasma after 7 days of depletion on a selenium-deficient (0.04 ppm of Se) diet. Selenium-depleted chicks fed graded levels of selenium (0.02-0.10 ppm in steps of 0.02 ppm) for 9 days had glutathione peroxidase activity in blood plasma which was linearly,highly correlated with dietary selenium content. In three subsequent experiments, chicks were fed a selenium-deficient diet for 7 days and were thendistributed into groups of five chicks each. The standard diets, which contained 0.02, 0.04, 0.06, 0.08 and 0.10 ppm of selenium added as NaHSeO3,and the test diets, which contained three levels of each test substance andprovided selenium supplements within the standard range, were each fed to three groups of chicks for 9 days. The dose parameter was selenium,in the standard groups added as NaHSeOs and in the test groups provided by the selenium present in the test substance. The response parameter was blood plasma glutathione peroxidase activity. Statistical analyses of theresults utilizing four fish meals as test substances revealed that the assaycomplied with the requirements for statistical and fundamental validity.J. Nutr. 110: 1089–1095,1980.INDEXING KEY W

KEY WORDS: • selenium • glutathione peroxidase • availability assay • blood plasma • chick

¹ The work reported in this paper was submitted as part of B. O. Gabrielsen's cand. real. dissertation, Bergen University, 1978.

² This work was partly supported by grants from the Norwegian Council for Fisheries Research.

Manuscript received 24 September 1979.