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Effects of -Pyridoxyllysine Bound to Dietary Protein on the Vitamin B-6 Status of Rats^1,2,3,

Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611

The nutritional properties of protein bound -pyridoxyllysine residues in a phosphopyridoxyl bovine serum albumin (PP-BSA) preparation were examined by rat bioassay employing various levels of PP-BSA and pyridoxine (PN) fortification in the diets. Previous studies have shown that vitamin B-6 aldehydes can reductively bind to food proteins as -pyridoxyllysine complexes during processing and storage. The bound pyridoxyllysine was found to possess 50% molar vitamin B-6 activity, as indicated by slope ratios for rat growth, feed efficiency and liver pyridoxal 5'-phosphate concentration. The response curves indicated that high dosages of -pyridoxyllysine would fully satisfy the vitamin B-6 requirement. Direct antivitamin B-6 activity of -pyridoxyllysine was observed in diets containing low levels of PP-BSA. Classical rat acrodynia symptoms, depression of erythrocyte aspartate aminotransferase (AspAT) activity and elevation of in vitro coenzyme stimulation of AspAT were observed to be inversely related to PP-BSA concentration in the diet. The antivitamin B-6 effects were readily prevented by the presence of added 0.5–1.0 µg pyridoxine/g diet. In contrast to the previous study, the presence of PP-BSA in the diet did not inhibit the utilization of added PN. This difference was postulated to be due to a difference in vitamin B-6 concentration of the basal diets. The results of this study indicate that protein bound -pyridoxyllysine is nutritionally significant, presumably by its participation in the normal metabolism of vitamin B-6. The metabolic implications are discussed.

KEY WORDS: • -pyridoxyllysine • vitamin B-6 • antivitamin

¹ Florida Agricultural Experiment Stations Journal Series Number 2024.

² This research was supported by funds from the Florida Agricultural Experiment Station.

³ Presented in part at the Third Joint Meeting of the American Institute of Nutrition, American Society for Clinical Nutrition and the Nutrition Society of Canada. Guelph, Ont., 5–7 July 1979.

Manuscript received 24 October 1979.

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