QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Qureshi, A. A. Articles by Sunde, M. L. Search for Related Content PubMed PubMed Citation Articles by Qureshi, A. A. Articles by Sunde, M. L.

Suppression of Cholesterol and Stimulation of Fatty Acid Biosynthesis in Chicken Livers by Dietary Cereals Supplemented with Culture Filtrate of Trichoderma viride^1,2,

Asaf A. Qureshi, Warren C. Burger, Neville Prentice, Herbert R. Bird^* and Milton L. Sunde^*

USDA, SEA, Barley and Malt Laboratory, 501 N. Walnut St. Department of Agronomy, University of Wisconsin, Madison, WI 53705 ^* Department of Poutry Science, University of Wisconsin, Madison, WI 53706

The levels of activity of ß-hydroxy-ß-methylglutaryl-CoA (HMG-CoA) reductase, acetyl-CoA carboxylase, fatty acid synthetase and the rate of conversion of [2-¹⁴C]acetate and mevalonate to nonsaponifiable compounds and digitonin-precipitable sterols were measured in subcellular fractions of livers of chickens fed diets supplemented with various cereals with and without solids from culture filtrate of Trichoderma viride. The chickens that were fed barley, oats or rye showed lower body and liver weights (31–39%) and lower levels of hepatic HMG-CoA reductase activity (up to 62–79%) than those fed corn (control diet). Marked differences in liver and plasma cholesterol were also noted, especially with barley. When diets were supplemented with the culture filtrate, low body weights were corrected and cholesterol synthesis was suppressed even more by inhibiting HMG-CoA reductase and conversion of acetate and mevalonic acid to cholesterol. Chickens fed barley and fungal product (0.008%) had 90% lower cholesterol synthesis than controls. Plasma and liver cholesterol were decreased when the culture filtrate was added to the other cereal diets. Acetyl-CoA carboxylase and fatty acid synthetase showed a two to sixfold increase with cereals and three to 11-fold with cereal plus culture filtrate-supplemented diets. The drastic decreases of cholesterol biosynthesis observed indicate the presence of inhibitor(s) in T. viride culture filtrate, which may provide insight into the control mechanism of cholesterol biosynthesis.

KEY WORDS: • chicks • cereals • culture filtrate of T. viride • cholesterol • fatty acids • liver enzymes • acetyl-CoA carboxylase • HMG-CoA reductase

¹ Cooperative investigation between the Science and Education Administration, U.S. Department of Agriculture, and College of Agricultural and Life Sciences. University of Wisconsin. Madison. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.

² A preliminary report of this work was presented at the XIth International Congress of Biochemistry, Toronto, Canada, 8–13 July 1979, abs, 04-2-S30, p. 256.

Manuscript received 30 November 1979.