Journal of Nutrition OpenSOurce Diets- www.ResearchDiets.com

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 110 No. 12 December 1980, pp. 2497-2505
Copyright © 1980 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Corwin, L. M.
Right arrow Articles by Shloss, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Corwin, L. M.
Right arrow Articles by Shloss, J.

Role of Antioxidants on the Stimulation of the Mitogenic Response1

Laurence M. Corwin and Janet Shloss

Department of Microbiology, Boston University School of Medicine, Boston, MA 02118

By comparing the activities of tocopherol with other anti-oxidants and tocopherol analogs on mitogenesis of murine spleen cells, an attempt was made to understand the mechanism of tocopherol stimulation. N,N'-diphenyl-p-phenylene diamine (DPPD) was very active in this system, whereas butylated hydroxytoluene, another antioxidant, was much less active. Trolox C, an analog of {alpha}-tocopherol with a carboxyl group instead of the isoprene side chain was totally inactive. Tocopherol quinone, an oxidized form of tocopherol, was almost as active as tocopherol. Menadione (vitamin K-3), a quinone without a side chain, was also active. It was suggested therefore, that although in tocopherol-like compounds the side chain seems important, the activities of DPPD and menadione clearly do not require it. On the other hand, the stimulation by tocopherol quinone and menadione appears to rule out a requirement for an antioxidant function in mitogenic stimulation. A comparison of the action of tocopherol with indomethacin ruled out regulation of the peroxidation step leading to prostaglandin synthesis as the mechanism for the tocopherol effect.

KEY WORDS: • {alpha} tocopherol • tocopherol analogs • antioxidants • mitogenesis • calcium

1 Supported by N1H grant AM21618 and a grant-in-aid from Hoffman-LaRoche Inc., Nutley, NJ.

Manuscript received 8 February 1980.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]