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Differential Regulation of Lipase and Colipase in the Rat Pancreas by Dietary Fat and Proteins

Centre de Recherches sur la Nutrition du C.N.R.S., 9, rue Jules Hetzel 92 190 Meudon-Bellevue, France

The adaptative response of the exocrine pancreas to diets rich in lipids and the influence of dietary proteins are reconsidered taking into account colipase. This was particularly necessary in the rat since colipase is often limiting with respect to lipase in this species. In a first experiment, adult male rats received diets containing 10, 15, 18, 40 or 86% casein together with 2, 8 or 40% lard. Under these conditions, lipase was found to be sensitive to variations in the lipid contents of the diet and was two-fold higher with 40% than with 8% lipid, in diets containing 40% casein. Proteins exerted a permissive effect since no response was recorded with diets containing less than 18% casein. Colipase responded to protein intake, even in lipid-poor diets (2% lard), and was increased by a factor 3 when casein was raised from 18 to 40%. In a second experiment, proteins were fed as a separate meal, and the remainder of the diet (provided ad libitum) contained either 8 or 40% lard. In a diurnal study, lipase and colipase were followed every 3 hours over 24 hours. Both lipase and colipase were found to accumulate after the protein meal. Colipase was found to accumulate much faster than lipase in all cases and reach 3 times basal levels 9 hours after the protein meal. This resulted in important diurnal variations in the ratio of colipase to lipase which modulates lipolytic activity. It is concluded that colipase is particularly sensitive to protein intake, perhaps more than to lipid intake and may become a limiting factor of lipid digestion.

KEY WORDS: • pancreatic enzymes • lipase • colipase • dietary fat • dietary protein

Manuscript received 3 April 1980.