Journal of Nutrition OpenSOurce Diets- www.ResearchDiets.com

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 110 No. 11 November 1980, pp. 2297-2301
Copyright
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sugarman, B.
Right arrow Articles by Munro, H. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sugarman, B.
Right arrow Articles by Munro, H. N.

[14C]-Pantothenate Accumulation by Isolated Adipocytes from Adult Rats of Different Ages

Barrett Sugarman1 and Hamish N. Munro2

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, MA 02139 and USDA Human Nutrition Center at Tufts University, Boston, MA

Accumulation of radiolabeled pantothenate by isolated adult rat adipocytes was studied in an in vitro system. Maximal uptake after 1 hour incubation occurred in media containing 0.03 mM pantothenate, about 100 picograms (pg) per adipocyte and was less than 1 pg per cell. Using such saturation conditions, uptake was energy- and temperature-dependent, with about 50% maximal accumulation occurring after 5–10 minutes incubation. Uptake was decreased by the addition of unlabeled pantothenate, but not other water-soluble vitamins. The serum level of pantothenate was decreased about 25% in animals 23–25 months old compared to those 11–13 months old, yet the ability to accumulate pantothenate at saturation conditions by adipocytes was no different. These data suggest that cellular accumulation of this vitamin is specific and that the capacity to do so under optimal conditions does not change with advancing age in this system. It is not known whether in vivo suboptimal or altered rate of cellular pantothenate uptake occurs with aging, or if it remains unchanged and lower serum levels are the result of decreased dietary intake.

KEY WORDS: • pantothenate • adipocytes • serum pantothenate • accumulation

1 Supported by a USPHS Training Grant in Clinical Nutrition.

2 To whom reprint requests should be sent at: Department of Nutrition and Food Science, 56-225 M.I.T., Cambridge, MA 02139.

Manuscript received 10 March 1980.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]