Journal of Nutrition Animal Diets/Enrichment Products...

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 110 No. 10 October 1980, pp. 2065-2077
Copyright © 1980 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Struthers, B. J.
Right arrow Articles by Hopkins, D. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Struthers, B. J.
Right arrow Articles by Hopkins, D. T.

Excretion and Tissue Distribution of Radioactive Lysinoalanine, N{varepsilon}-DL-(2-Amino-2-Carboxyethyl)-U-14C-L-Lysine (LAL) in Sprague-Dawley Rats1

Barbara J. Struthers, Janice R. Brielmaier, Marvin L. Raymond, Robert R. Dahlgren and Daniel T. Hopkins

Central Research, Ralston Purina Co., St. Louis, MO

Rats were fed either a control soybean protein diet or a diet containing 3,000 ppm soybean protein-bound lysinoalanine (LAL) for 4 or 6 weeks, at which time all rats were dosed by stomach tube with 14C-LAL labeled in the lysine moiety. Urinary and fecal excretion and tissue distribution were followed in one experiment at 6, 12, 18, 24, 48 and 72 hours. Excretion in urine, feces and expired air was followed in the other metabolic experiment at 2-hour intervals for 48 hours, and at 24-hour intervals for the next 7 days. Tissue samples were counted and LAL determination was made by amino acid analysis in both experiments. The group of rats fed LAL excreted slightly more LAL than the group fed the control diet. Very little LAL remained in the rat tissues after either experiment, and the largest remaining quantity of radioactivity was found as lysine. Quantitation of 14C-lysine in the original material and in the material from rat organs showed that the rat has some capacity for converting LAL to lysine. Less than 0.5% of the original 14C remained in any organ examined 9 days after dosing in either control or LAL-fed rats. Autoradiographs of the kidneys 24 hours after dosing showed that the radioactive material had accumulated in the proximal convoluted tubules of the corticomedullary junction.

KEY WORDS: • lysinoalanine • excretion • tissue distribution • autoradiography

1 Presented in part at FASEB, 1977, and at the Keystone conference (Soy protein in human nutrition, June 1978, Keystone, CO).

Manuscript received 1 February 1980.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]