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An Improved Synthetic Ration for Vitamin B₄ Studies¹

One Figure

Department of Agricultural Chemistry, University of Wisconsin, Madison

1. A revised method for the study of vitamin B₄ in chicks has been described.

2. Changes in a synthetic ration for these studies, previously described, include purification of the casein and use of highly potent sources of the factors in the B complex other than B₄.

3. It was found necessary to add to the ration a substance which prevents the occurrence of lesions in the lining of the gizzard of the experimental chicks. Dried lung tissue is a potent source of this ‘gizzard factor.’.

4. On the improved basal synthetic ration a nutritional paralysis occurs in 100 per cent of the animals, which may be prevented by addition of supplements such as peanuts, brain, kidney or liver tissue.

Manuscript received 31 January 1936.