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The Use of 3-Day Periods in Human Metabolism Studies¹

Calcium and Phosphorus

Two Figures

Samuel S. Fels Research Foundation, Antioch College, Yellow Springs, Ohio

A comparison was made between twelve calcium and phosphorus balances computed by the 3-day method and those given by Macy and her co-workers ('30), and twelve from each of the studies made by Coons and her co-workers ('30, '34). The balances in the four studies were matched chronologically in order to rule out differences seen at various stages in pregnancy due to the growth of the fetus and changes in maternal tissues.

The results from 24-hour balance sampling preceded by 2 days of preparation when the subject ate the same kind and as near the same quantity of food as on the third (collecting) day did not differ significantly enough from the results given in the other three studies to indicate that a period of this length fails to show an individual's metabolic tendencies during pregnancy.

The findings point toward greater individual differences than difference due to method.

On the strength of these indications we feel that the 3-day balance period with a 24-hour sampling on the third day merits consideration as a clinical tool in determining human calcium and phosphorus utilization during pregnancy.

Manuscript received 21 January 1936.