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myo-Inositol Deficiency: Studies on the Mechanism of Lactation-Dependent Fatty Liver Formation in the Rat¹

Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824

Intravenous administration of [1-¹⁴C]palmitate to 8-day lactating rats fed a myo-inositol supplemented or deficient diet resulted in rapid labeling of liver triglycerides and phospholipids. Compared with myo-inositol deficient rats, those supplemented with myo-inositol showed a greater loss of isotope from liver triglycerides with a more rapid appearance of isotope in serum triglyceride. Loss of ¹⁴C from liver phospholipids was similar for both groups, whereas the appearance of labeled phospholipids in serum was slightly greater for myo-inositol supplemented controls compared with myo-inositol deficient rats. The labeling pattern of liver microsomal triglycerides and phospholipids of the two dietary groups was similar; however, liver microsomal protein was significantly reduced in myo-inositol deficient rats relative to the control group. Concurrent administration of Triton WR-1339 with [1-¹⁴C]palmitate resulted in significantly less label accumulation in serum triglycerides of myo-inositol deficient rats compared with myo-inositol supplemented rats. Labeled triglycerides in whole liver and in liver microsomes of myo-inositol supplemented rats turned over more rapidly than those of myo-inositol deficient rats while no significant difference was noted for the [¹⁴C]palmitate labeled phospholipid of either source. The incorporation of [guanido-¹⁴C]arginine into total liver and serum protein 1 hour after injection of the precursor was similar whether the 14-day lactating dams were myo-inositol supplemented or deficient, but total serum protein specific radioactivity of myo-inositol deficient rats was 66% that of myo-inositol supplemented rats. Thus, the significantly reduced release of hepatic triglycerides appears to be the cause of the fatty liver observed during lactation-dependent myo-inositol deficiency.

KEY WORDS: • myo-inositol deficiency • rat • fatty liver • triglyceride • phospholipid • Triton • palmitate • microsomes • serum • liver • intestine

¹ This work was supported by Grant HD06007, U.S. Public Health Service, Michigan Agricultural Experiment Station Journal Article No. 8901.

² Present address: Department of Neurobiology, Stanford Medical School, Stanford, California (CA) 94305.

³ To whom inquiries should be addressed.

Manuscript received 12 February 1979.