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The Nutritive Value of Dehulled Soybeans Fermented with Aspergillus Oryzae or Rhizopus Oligosporus as Evaluated by Rats¹

110 Animal Science Research Center, University of Missouri, Columbia, Missouri 65211

Two 28-day experiments involving 258 growing rats were conducted to compare the nutritive value of heated unfermented dehulled soybeans (HUS), heated dehulled soybeans fermented with Aspergillus oryzae (AOS) or heated dehulled soybeans fermented with Rhizopus oligosporus (ROS). The diets contained 20.26 and 14.89% of the various soybean products in experiments 1 and 2, respectively. Thus, the diets contained approximately 9.0 and 6.7% crude protein in experiments 1 and 2, respectively. Proximate analyses of the above soybean products were similar even though a 3% loss in dry matter resulted during the fermentation procedure. The percentages of the various amino acids either remained unchanged or were not changed by more than ± 10% as a result of fermentation. Average daily gain (ADG) and gain:feed (G:F) for the 4-week period were greater for the rats fed diets containing AOS or ROS compared to rats fed the diet containing HUS for both experiments. There was a 30% improvement in ADG due to fermentation of the soybeans in experiment 2, while in experiment 1 only a 12% improvement was observed. Serum urea nitrogen and serum total protein were similar for all three dietary groups as were the pancreas weights determined on day 28. The diets containing AOS or ROS had a greater apparent biological value and apparent net protein utilization when fed to rats compared to the diets containing HUS. However, the digestible energy utilization was similar among the treatment groups. The greater apparent net protein utilization appearéd to be responsible for the improved performance obtained by the rats fed the diets containing AOS or ROS compared to rats fed the diet containing HUS.

KEY WORDS: • fermented soybeans • Aspergillus oryzae • Rhizopus oligosporus • growing rats • protein utilization

¹ Contribution from the Missouri Agricultural Experiment Station.

Manuscript received 17 July 1978.