Journal of Nutrition

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Journal of Nutrition Vol. 109 No. 5 May 1979, pp. 904-915
Copyright © 1979 by American Society for Nutrition
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Determination of Intravenous Non-Protein Energy and Nitrogen Requirements in Growing Rats

Robert C. Tao, Norman N. Yoshimura, Irene B. Chinn and Allan M. Wolfe

McGaw Laboratories, 2525 McGaw Avenue, Irvine, California 92714

Two experiments were conducted to estimate total nonprotein energy and nitrogen requirements in growing healthy male Wistar rats nourished by parenteral nutrition. In experiment 1, non-protein energy varying from 30 to 70 kcal/day/rat were administered to animals receiving a constant dose of 80 mg nitrogen, plus vitamins and minerals. In experiment 2, nitrogen dosages varying from 0 to 280 mg N/day/rat with a constant dose of 60 kcal non-protein energy were studied. The formulation of the amino acid solution used in both experiments was based upon recommended oral amino acid requirements for growing rats. Dextrose served as the source of non-protein energy. Weight gain and nitrogen balance during a 6-day experimental period were used to determine requirements. Plasma free amino acids were also analyzed to evaluate the amino acid solution. Results indicate that under total parenteral nutrition conditions 578 to 621 mg/kg body weight3/4 nitrogen and 171 to 182 kcal/kg body weight3/4 non-protein energy are required to achieve growth of approximately 3 g/day. Inconsistent responses of plasma amino acid concentrations to the amounts infused were observed. It is suggested that the determined requirements can be applied as guidelines to research using the rat as an animal model in total parenteral nutrition.

KEY WORDS: • total parenteral nutrition • amino acids • nitrogen requirement • energy requirement • rat

Manuscript received 15 May 1978.




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