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Effect of Lactose on Duodenal Calcium-Binding Protein and Calcium Absorption¹

Laboratoire de Pathologie et Thérapeutique des Echanges Minéraux Ecole Pratique des Hautes Etudes, Pavillon H, Hôpital E. Herriot 69374 Lyon Cedex 2, France, and Department of Oral Biology, The University of Connecticut Health Center, Farmington, Connecticut 06032 USA

Rats were fed a purified diet containing 30% lactose and calcium absorption was measured in duodenal loops in situ following instillation of 1.25 or 10 mM CaCl₂ solutions. Lactose feeding caused absorption to be depressed from 88 to 69% (1.25 mM Ca solution) and from 71 to 43% (10 mM Ca solution). The effect of lactose feeding was more pronounced in 5-month old rats than in 2-month old rats. In the lactose-fed rats, calcium-binding protein (CaBP), measured by a competitive binding assay following partial purification, was depressed on the average from 24 to 10 nmoles Ca bound per mg protein. The effect of the lactose ingestion can be likened to the effect expected from continued high calcium intake, i.e., a decrease in the efficiency of calcium absorption and a decrease in CaBP.

KEY WORDS: • calcium-binding protein • intestinal absorption • lactose • effect of age

¹ This work was carried out under contract n^o 1908. Action Thématique Programmée du CNRS. Physiologie et Pathologie du Tissu Calciné.

Manuscript received 15 May 1978.