Journal of Nutrition Animal Diets/Enrichment Products...

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 108 No. 8 August 1978, pp. 1343-1347
Copyright © 1978 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Canfield, L. M.
Right arrow Articles by Chytil, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Canfield, L. M.
Right arrow Articles by Chytil, F.

Effect of Low Lysine Diet on Rat Protein Metabolism1

Louise M. Canfield2 and Frank Chytil

Departments of Biochemistry and Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Prolonged lysine deficiency resulted in inhibition of growth of the rat and in lowered levels of albumin and ß-globulins, but not of total liver proteins. The apparent incorporation of [3H]lysine into total and chromosomal liver proteins, however, was increased in lysine deficient rats relative to pair-fed controls. The concentration of free lysine in neither serum nor liver was altered in lysine deficient rats. The specific radioactivity of liver free lysine 2 hours after administration of [3H]lysine was noticeably elevated in the deficient rats. However, when the pool of liver free lysine is considered, synthesis of all proteins studied was inhibited. The largest inhibitory effect was seen in serum albumin and total liver protein synthesis. Inhibition of synthesis of nuclear chromatin protein was less pronounced. The data indicate an adaptive mechanism operates to preserve liver lysine in rats fed lysine deficient diets for a prolonged period. In spite of retention of lysine, the synthesis of liver and serum proteins was inhibited.

KEY WORDS: • lysine deficiency • plasma proteins • incorporation • lysine pool

1 Supported by U.S. Public Health Service Grants No. HD-05384, HD-09195, HL-1541, and AM-07083.

2 Present address: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706.

Manuscript received 27 June 1977.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]