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Effects of Starvation and Molecular Weight on the Turnover of Soluble Proteins from Five Tissues of the Japanese Quail¹

Department of Animal Science, The University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2

Three groups of four Japanese quail were assigned to fed ad libitum, 3-day starved and 10-day starved treatments. Three days prior to the conclusion of each treatment period, each bird was given a measured quantity of [1-¹⁴C]leucine and 4 hours prior to the end each bird was given a measured quantity of [4,5-³H]leucine. Tissues were collected and the amount of ³H and ¹⁴C was measured in the soluble proteins from composite samples of each tissue in each treatment group after they had been separated on Sephadex G-200. The ratio of ³H to ¹⁴C was determined and this was related to protein half-life. Starvation caused the ³H:¹⁴C ratios of liver, kidney, brain, and heart muscle soluble proteins to decrease (which is interpreted as increased half-lives) and ³H:¹⁴C ratios of breast muscle soluble proteins to increase (which is interpreted as decreased half-lives). The ³H:¹⁴C ratios of the soluble proteins from all tissues became greater as the protein molecular weight became larger. Comparing the soluble proteins from those tissues tested, it was found that as the tissue ³H:¹⁴C ratio became larger, the difference in ³H:¹⁴C ratio between the large and small proteins also became larger. Starvation decreased the ³H:¹⁴C ratio in four of the tissues tested and also decreased the difference in ³H:¹⁴C ratio between the large and small soluble proteins. Some of the implications of starvation on protein turnover are discussed.

KEY WORDS: • protein turnover • soluble protein • quail tissues • ³H:¹⁴C ratios • starvation

¹ This work was supported by a grant from the National Research Council of Canada.

² Present address, Research Station, Agriculture Canada, P.O. Box 20280, Fredericton, New Brunswick, Canada, E3B 4Z7.

Manuscript received 9 May 1977.