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Zinc Absorption and Metabolism by Isolated, Vascularly Perfused Rat Intestine^{1, 2,}

Department of Nutrition, Rutgers—The State University of New Jersey, New Brunswick, New Jersey 08903

An isolated, vascularly perfused rat intestine system was utilized to examine various aspects of zinc absorption in an attempt to more clearly examine the mechanisms involved. The lumen was perfused with a modified tissue culture medium containing ⁶⁵Zn. The vascular system was perfused from the superior mesenteric artery to the portal vein, with Krebs-Ringer bicarbonate buffer containing 5% rat serum. The criterion for absorption was the amount of radioactivity transferred to the vascular perfusate. When the intestines were obtained from rats that had consumed a zinc-deficient diet the amount of zinc absorbed increased markedly. Conversely, elevation of zinc status decreased the amount of ⁶⁵Zn that could be transferred to the vascular perfusate. These data strongly suggest that the isolated, perfused rat intestine retains the ability to exercise homeostatic control over ⁶⁵Zn absorption. Transfer of infused ⁶⁵Zn to the vascular perfusate was significantly decreased by aspirin, phytate, and prostaglandin E₂. Uptake of ⁶⁵Zn from the lumen into the intestinal cells was significantly increased by histidine and significantly decreased by phytate and prostaglandin E₂. Thus, the isolated, vascularly perfused rat intestine appears to be capable of differentiating between the cellular uptake and cell to plasma transfer phases of zinc absorption.

KEY WORDS: • zinc • absorption • intestinal perfusion • metabolism

¹ This work was supported by Grant No. AM18555 from the National Institute of Arthritis, Metabolism and Digestive Diseases, DHEW, and Grant No. ES00777 from the National Institute of Environmental Health Sciences, DHEW.

² Presented in part at the American Institute of Nutrition Meeting. Atlantic City, New Jersey, April 1978. Abstract published in Fed. Proc. 37: abs. #643.

³ Author to whom reprint requests should be addressed.

⁴ Present Address: College of Veterinary Medicine, Ohio State University, Columbus, Ohio 48210.

Manuscript received 12 June 1978.

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