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Lactate as a Precursor of Fatty Acids in Bovine Adipose Tissue¹

Departments of Animal Science and Biochemistry-Biophysics, Iowa State University, Ames, Iowa 50011

In vitro experiments were conducted to determine the rates of lactate, acetate, and glucose conversions to glycerol, CO₂, and fatty acids by subcutaneous adipose tissue of cattle. To study the pathway for lactate utilization as a carbon source for lipogenesis, the effect of each of four substrates (lactate, acetate, pyruvate, and glucose) on the rate of conversion of the other three was determined. The rates of lactate conversion to fatty acids and to glycerol were greater than those for acetate and glucose. Acetate, however, was oxidized to CO₂ at greater rates than either lactate or glucose. Acetate was used for fatty acid synthesis at greater rates than pyruvate and glucose. The rate of fatty acid synthesis from pyruvate was less than that from lactate but greater than that from glucose. Acetate additions to the incubation media decreased the synthetic rates from lactate, pyruvate, and glucose. Pyruvate decreased rates of fatty acid synthesis from glucose but slightly (not statistically significant) stimulated synthesis from lactate and acetate. Lactate decreased glucose and pyruvate utilization but had no effect upon that of acetate. Our results clearly demonstrated that lactate can be used for fatty acid synthesis as well as for glycerogenesis by bovine adipose tissue.

KEY WORDS: • cattle • lipogenesis • lactate • adipose tissue

¹ Journal Paper No. J-9067 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Project No. 2187. Part of this study was presented at the annual meeting of the American Institute of Nutrition, Anaheim, California, April, 1976. Fed. Proc. 35, 580 (1976) (Abstr.)

² Present address: Moorman Mfg. Co., Quincy, Illinois 62301.

³ Present address: University of Iowa Medical School. Iowa City, Iowa 52240.

⁴ Department of Veterinary Anatomy, Pharmacology. Physiology, Iowa State University, Ames, Iowa 50011.

Manuscript received 6 March 1978.

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