Journal of Nutrition

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Journal of Nutrition Vol. 108 No. 10 October 1978, pp. 1616-1620
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Effect of Dietary Vitamin E and Vitamin C on Respiration and Swelling of Guinea Pig Liver Mitochondria

L. H. Chen and M. L. Chang1,2,

Department of Nutrition and Food Sciences, University of Kentucky, Lexington, Kentucky 405061

The effect of dietary vitamin C on vitamin E status and function was studied with weanling male Hartley guinea pigs. Thirty guinea pigs fed a basal diet free of vitamins E and C were divided into two groups; only one group received 2 mg/100 g body weight/day of vitamin C. Each group was further divided into three subgroups and received 0, 0.2, and 0.4 IU/100 g body weight/day of vitamin E. After 5 weeks the animals were killed. Degree of erythrocyte hemolysis induced by dialuric acid, level of plasma vitamin E and extent of liver lipid peroxidation were determined. Liver mitochondria were isolated and in vitro oxygen uptake was measured. The rate of swelling of mitochondria in two media was also determined. The results showed that the adequate dietary vitamin C supplementation did not affect the extent of erythrocyte hemolysis induced by dialuric acid, but increased the plasma vitamin E level and lowered liver lipid peroxidation. It also maintained the normal respiration and swelling rates of liver mitochondria when vitamin E was inadequate.

KEY WORDS: • vitamin E • vitamin C • mitochondria • respiration • swelling • guinea pigs

1 Supported by Kentucky Agricultural Experiment Station, Paper No. 78-9-139.

2 Part of the data taken from a thesis submitted by Mei-ling Chang in partial fulfilment of the requirements for the degree of Master of Science.

Manuscript received 22 February 1978.




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